gravity column (Sangon Biotech)
86
Structured Review
Sangon Biotech
gravity column
Gravity Column, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gravity+column/column+gravity/pm42288757-121-15-17
Average 86 stars, based on 1 article reviews
Gravity Column, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/gravity+column/column+gravity/pm42288757-121-15-17
Average 86 stars, based on 1 article reviews
gravity column - by Bioz Stars,
2026-09
86/100 stars
Images
Related Articles
Transfection:Article Title: Antibody and bispecific antibody targeting LAG-3 and use thereof Article Snippet: .. The transfected cells were incubated for 5 days in a shaker at 37° C. in 8% CO2, and then the supernatant was collected and purified after centrifuging at 3300 g for 10 min. Purification of antibodies or -Fc fusion proteins: the samples were centrifuged at high speed to remove impurities, and a Incubation:Article Title: Antibody and bispecific antibody targeting LAG-3 and use thereof Article Snippet: .. The transfected cells were incubated for 5 days in a shaker at 37° C. in 8% CO2, and then the supernatant was collected and purified after centrifuging at 3300 g for 10 min. Purification of antibodies or -Fc fusion proteins: the samples were centrifuged at high speed to remove impurities, and a Purification:Article Title: Antibody and bispecific antibody targeting LAG-3 and use thereof Article Snippet: .. The transfected cells were incubated for 5 days in a shaker at 37° C. in 8% CO2, and then the supernatant was collected and purified after centrifuging at 3300 g for 10 min. Purification of antibodies or -Fc fusion proteins: the samples were centrifuged at high speed to remove impurities, and a Article Title: Multi-strategy engineering of Saccharomyces cerevisiae for enhanced β-ionone biosynthesis Article Snippet: β-Ionone is a natural volatile compound known for its distinctive violet and woody aroma, with broad application potential in the flavor, fragrance, and pharmaceutical industries.. To meet the growing market demand for natural products and overcome the limitations of chemical synthesis and plant extraction, microbial cell factories have emerged as a sustainable and environmentally friendly alternative.. In this study, a positive correlation was established between β-ionone production and the accumulation of its precursor, β-carotene. Article Title: The Cloning and Expression of Trypsins from Yellow Mealworm (Tenebrio molitor) Article Snippet: Seven non-redundant trypsin genes including two sequences without signal peptide (TMT2 and TMT5) and five sequences with signal peptide (pTMT2–pTMT6) were screened from yellow mealworms survived from stress of Bt toxin.. Three amino acid variations were unique to TMT/pTMT, which were the substitution of I87T from pTMT6, and the substitutions of S29P and N205D from TMT2.. The substitution of S29P caused the terminal of a β-sheet to transform into coil. Article Title: Telomere-to-telomere genome assembly of Metarhizium acridum provides insights into Metarhizium genome evolution. Article Snippet: .. The proteins were purified using a protein purification kit, HyPur T Ni-NTA 6FF (His-Tag) PrePacked Article Title: USP7 V517F mutation as a mechanism of inhibitor resistance. Article Snippet: Thepellet was then resuspended in lysis buffer (50mM HEPES, 300mMNaCl, 10mM Imidazole, 3mM β-Mercaptoethanol, pH 7.5) and sonicated for lysis.After centrifugation to clarify the lysate, the supernatant was applied to a Ni–NTA gravity column (Beyotime, Shanghai, China), washed with lysis buffer containing 100mM Imidazole, and elutedwith 250mM Imidazole. .. Theproteinwas concentrated using anAmiconUltra-15 Centrifugal Filter (MerckMillipore; Germany), purified by desalting through a Centrifugation:Article Title: Identification and characterization of RacX, a new broad-specificity amino acid racemase from a novel taxon within the order Flavobacteriales . Article Snippet: .. Next, the lysates obtained were subjected to centrifugation at 12,000 × g for 20 min at 4°C, and the supernatant was subjected to immobilized metal affinity column chromatography with the HyPur T Ni-IDA 6FF (His-Tag) PrePacked Article Title: Identification and characterization of RacX, a new broad-specificity amino acid racemase from a novel taxon within the order Flavobacteriales Article Snippet: .. Next, the lysates obtained were subjected to centrifugation at 12,000 × g for 20 min at 4°C, and the supernatant was subjected to immobilized metal affinity column chromatography with the HyPur T Ni-IDA 6FF (His-Tag) PrePacked Affinity Column:Article Title: Identification and characterization of RacX, a new broad-specificity amino acid racemase from a novel taxon within the order Flavobacteriales . Article Snippet: .. Next, the lysates obtained were subjected to centrifugation at 12,000 × g for 20 min at 4°C, and the supernatant was subjected to immobilized metal affinity column chromatography with the HyPur T Ni-IDA 6FF (His-Tag) PrePacked Article Title: Identification and characterization of RacX, a new broad-specificity amino acid racemase from a novel taxon within the order Flavobacteriales Article Snippet: .. Next, the lysates obtained were subjected to centrifugation at 12,000 × g for 20 min at 4°C, and the supernatant was subjected to immobilized metal affinity column chromatography with the HyPur T Ni-IDA 6FF (His-Tag) PrePacked Chromatography:Article Title: Identification and characterization of RacX, a new broad-specificity amino acid racemase from a novel taxon within the order Flavobacteriales . Article Snippet: .. Next, the lysates obtained were subjected to centrifugation at 12,000 × g for 20 min at 4°C, and the supernatant was subjected to immobilized metal affinity column chromatography with the HyPur T Ni-IDA 6FF (His-Tag) PrePacked Article Title: Identification and characterization of RacX, a new broad-specificity amino acid racemase from a novel taxon within the order Flavobacteriales Article Snippet: .. Next, the lysates obtained were subjected to centrifugation at 12,000 × g for 20 min at 4°C, and the supernatant was subjected to immobilized metal affinity column chromatography with the HyPur T Ni-IDA 6FF (His-Tag) PrePacked Recombinant:Article Title: The Cloning and Expression of Trypsins from Yellow Mealworm (Tenebrio molitor) Article Snippet: Seven non-redundant trypsin genes including two sequences without signal peptide (TMT2 and TMT5) and five sequences with signal peptide (pTMT2–pTMT6) were screened from yellow mealworms survived from stress of Bt toxin.. Three amino acid variations were unique to TMT/pTMT, which were the substitution of I87T from pTMT6, and the substitutions of S29P and N205D from TMT2.. The substitution of S29P caused the terminal of a β-sheet to transform into coil. Multiplex sample analysis:Article Title: The Cloning and Expression of Trypsins from Yellow Mealworm (Tenebrio molitor) Article Snippet: Seven non-redundant trypsin genes including two sequences without signal peptide (TMT2 and TMT5) and five sequences with signal peptide (pTMT2–pTMT6) were screened from yellow mealworms survived from stress of Bt toxin.. Three amino acid variations were unique to TMT/pTMT, which were the substitution of I87T from pTMT6, and the substitutions of S29P and N205D from TMT2.. The substitution of S29P caused the terminal of a β-sheet to transform into coil. Affinity Chromatography:Article Title: The Cloning and Expression of Trypsins from Yellow Mealworm (Tenebrio molitor) Article Snippet: Seven non-redundant trypsin genes including two sequences without signal peptide (TMT2 and TMT5) and five sequences with signal peptide (pTMT2–pTMT6) were screened from yellow mealworms survived from stress of Bt toxin.. Three amino acid variations were unique to TMT/pTMT, which were the substitution of I87T from pTMT6, and the substitutions of S29P and N205D from TMT2.. The substitution of S29P caused the terminal of a β-sheet to transform into coil. Protein Purification:Article Title: Telomere-to-telomere genome assembly of Metarhizium acridum provides insights into Metarhizium genome evolution. Article Snippet: .. The proteins were purified using a protein purification kit, HyPur T Ni-NTA 6FF (His-Tag) PrePacked other:Article Title: Identification, expression, and functional analysis of a chitin-degrading enzyme CtChi70 for N-acetyl Chitobiose production. Article Snippet: Chitin, the second most abundant natural polysaccharide, is a renewable resource with great potential for producing functional oligosaccharides.. In this study, we identified and characterized CtChi70, a glycoside hydrolase family 18 (GH18) exochitinase that selectively hydrolyzes chitin to produce N-acetyl chitobiose [(GlcNAc)2] with exceptional purity (≥98 %).. CtChi70 was heterologously expressed in Escherichia coli CtChi70 at a high yield of 228.2±47.8 mg L 1. Western Blot:Article Title: USP7 V517F mutation as a mechanism of inhibitor resistance. Article Snippet: Thepellet was then resuspended in lysis buffer (50mM HEPES, 300mMNaCl, 10mM Imidazole, 3mM β-Mercaptoethanol, pH 7.5) and sonicated for lysis.After centrifugation to clarify the lysate, the supernatant was applied to a Ni–NTA gravity column (Beyotime, Shanghai, China), washed with lysis buffer containing 100mM Imidazole, and elutedwith 250mM Imidazole. .. Theproteinwas concentrated using anAmiconUltra-15 Centrifugal Filter (MerckMillipore; Germany), purified by desalting through a Staining:Article Title: USP7 V517F mutation as a mechanism of inhibitor resistance. Article Snippet: Thepellet was then resuspended in lysis buffer (50mM HEPES, 300mMNaCl, 10mM Imidazole, 3mM β-Mercaptoethanol, pH 7.5) and sonicated for lysis.After centrifugation to clarify the lysate, the supernatant was applied to a Ni–NTA gravity column (Beyotime, Shanghai, China), washed with lysis buffer containing 100mM Imidazole, and elutedwith 250mM Imidazole. .. Theproteinwas concentrated using anAmiconUltra-15 Centrifugal Filter (MerckMillipore; Germany), purified by desalting through a |